Siglece^{tm1.1(SIGLEC16)Avrk}
Targeted Allele Detail

Summary

• Symbol: Siglece^{tm1.1(SIGLEC16)Avrk}
• Name: sialic acid binding Ig-like lectin E; targeted mutation 1.1, Ajit Varki
• MGI ID: MGI:6195824
• Synonyms: E16, Siglec-E16
• Gene: Siglece Location: Chr7:43300494-43309585 bp, - strand Genetic Position: Chr7, 28.25 cM, cytoband B2
• Alliance: Siglece^{tm1.1(SIGLEC16)Avrk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264224
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The targeting vector contains from 5' to 3' a loxP site, cDNA encoding the transmembrane and cytosolic domains of human SIGLEC16 gene, a human growth hormone polyadenylation sequence and an FRT-site flanked neo cassette inserted into exon 4. A second loxP site is inserted downstream of exon 5. Flp-mediated recombination removed the FRT-flanked neo cassette. The human SIGLEC16 activating receptor domain replaces the mouse inhibitory domain encoded by exon 4 of the mouse gene. Expression of human SIGLEC16 is detected in blood neutrophils and macrophages from the liver and spleen of mutant mice. (J:264224)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Siglece Mutation: 33 strains or lines available

References

• Original: J:264224 Schwarz F, et al., Paired Siglec receptors generate opposite inflammatory responses to a human-specific pathogen. EMBO J. 2017 Mar 15;36(6):751-760
• All: 2 reference(s)