Gt(ROSA)26Sor^{tm1(CAG-MYCN,-luc)Jhsc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-MYCN,-luc)Jhsc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Johannes H Schulte
• MGI ID: MGI:6196127
• Synonyms: LSL-MYCN
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-MYCN,-luc)Jhsc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222527
• Parent Cell Line: ART4.12 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, RMCE-ready, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-MYCN,-luc)Jhsc} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MYCN (4613)

  Mycn	(MGI:97357)
  Mycs	(MGI:1332242)

• Mutation details: Recombinase-mediated cassette exchange (RMCE) replaced the selection cassette in Gt(ROSA)26Sor^{tm1(CAG-flpe)Arte} with a 5' deleted neomycin resistance cassette, the chicken actin gene (CAG) promoter, loxP flanked strong transcriptional termination site (LSL), the human MYCN gene, IRES, and the firefly luciferase gene. Cre-mediated recombination can be utilized to remove the transcription termination site 5' to the MYCN allele to get MYCN expression. (J:222527)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Gt(ROSA)26Sor^{tm1(CAG-MYCN,-luc)Jhsc}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:222527 Althoff K, et al., A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies. Oncogene. 2015 Jun;34(26):3357-68
• All: 9 reference(s)