Klra1^tm1.1Wyok
Targeted Allele Detail

Summary

• Symbol: Klra1^tm1.1Wyok
• Name: killer cell lectin-like receptor, subfamily A, member 1; targeted mutation 1.1, Wayne M Yokoyama
• MGI ID: MGI:6196148
• Synonyms: AYF
• Gene: Klra1 Location: Chr6:130340881-130363837 bp, - strand Genetic Position: Chr6, 63.44 cM
• Alliance: Klra1^tm1.1Wyok page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:252851
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site flanked neomycin resistance gene cassette was inserted into intron 4. A substitution was engineered in exon 4 that changed codon 8 from tyrosine to phenylalanine (p.Tyr8Phe or p.Y8F). This mutation disrupts the cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) of the peptide, effectively creating a knock-out. The neo cassette was removed through subsequent cre-mediated recombination. (J:252851)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Klra1 Mutation: 17 strains or lines available

References

• Original: J:252851 Bern MD, et al., Immunoreceptor tyrosine-based inhibitory motif-dependent functions of an MHC class I-specific NK cell receptor. Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):E8440-E8447
• All: 2 reference(s)