Rbp4^tm2Zhel
Targeted Allele Detail

Summary

• Symbol: Rbp4^tm2Zhel
• Name: retinol binding protein 4, plasma; targeted mutation 2, Zhenghua Li
• MGI ID: MGI:6196354
• Synonyms: Rbp4^-
• Gene: Rbp4 Location: Chr19:38105077-38113729 bp, - strand Genetic Position: Chr19, 32.75 cM, cytoband D1
• Alliance: Rbp4^tm2Zhel page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260684
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout, RMCE-ready)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A null allele was created by replacing the ATG start site within exon 2 with a PGK-neo cassette flanked by lox71 and loxP sites. Besides disrupting the gene, the cassette also serves as an acceptor in recombination mediated cassette exchange (RMCE). RT-PCR confirmed absence of mRNA in the brain, lung, liver, and kidney. Northern blots of liver RNA and Western blots of serum confirmed absence of mRNA and protein, respectively. (J:260684)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbp4 Mutation: 21 strains or lines available

References

• Original: J:260684 Shen J, et al., Severe ocular phenotypes in Rbp4-deficient mice in the C57BL/6 genetic background. Lab Invest. 2016 Jun;96(6):680-91
• All: 3 reference(s)