Rubcn^em1Dgre
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rubcn^em1Dgre
• Name: RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; endonuclease-mediated mutation 1, Douglas R Green
• MGI ID: MGI:6196512
• Synonyms: Rubicon^-
• Gene: Rubcn Location: Chr16:32642072-32698121 bp, - strand Genetic Position: Chr16, 23.38 cM
• Alliance: Rubcn^em1Dgre page

Mutation origin

• Strain of Origin: (C57BL/6N x C57BL/6J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: Aspartic acid codon 178 in exon 5 was changed to premature stop codon TAA using an sgRNA and a 183 nt single-stranded DNA oligonucleotide template with CRISPR/Cas9 technology. Two additional substitutions were introduced immediately downstream to create an EcoRI restriction site for diagnostic purposes. The mutation is encoded NM_001200038.1:c.562_569delinsTAAGAAT. All necessary components were microinjected into the cytoplasm of C57BL/6 zygotes. (J:261225)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rubcn Mutation: 40 strains or lines available

References

• Original: J:261225 Martinez J, et al., Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins. Nat Cell Biol. 2015 Jul;17(7):893-906
• All: 14 reference(s)