Gt(ROSA)26Sor^{tm1(OVAL/fla,GFP)Vnce}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(OVAL/fla,GFP)Vnce}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Russell E Vance
• MGI ID: MGI:6196853
• Synonyms: iOvaFla
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(OVAL/fla,GFP)Vnce} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:260047
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A construct encoding a loxP-flanked transcriptional STOP cassette upstream of chicken ovalbumin lacking its signal sequence fused to the C-terminal 166 amino acids of Legionella pneumophila flagellin and an IRES-GFP downstream of the fusion gene was targeted to the locus. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette. Expression of flagellin activates endogenous NAIP/NLLRC4 inflammasome while the ovalbumin permits the tracking of the adaptive immune response. (J:260047)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 992 strains or lines available

References

• Original: J:260047 Nichols RD, et al., NAIP/NLRC4 inflammasome activation in MRP8(+) cells is sufficient to cause systemic inflammatory disease. Nat Commun. 2017 Dec 20;8(1):2209
• All: 2 reference(s)