Atad3a^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Atad3a^{tm1c(KOMP)Wtsi}
• Name: ATPase family, AAA domain containing 3A; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6197790
• Synonyms: Atad3^LoxP
• Gene: Atad3a Location: Chr4:155825098-155845550 bp, - strand Genetic Position: Chr4, 87.2 cM
• Alliance: Atad3a^{tm1c(KOMP)Wtsi} page
• IMPC: Atad3a gene page

Mutation origin

• Mutant Cell Line: EPD0159_4_A12
• Germline Transmission: Earliest citation of germline transmission: J:263681
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 155841261 of Chromosome 4 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 155842075. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by subsequent flp-mediated recombination to remove the reporter and neo cassettes. (J:263681)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atad3a Mutation: 30 strains or lines available

References

• Original: J:263681 Peralta S, et al., ATAD3 controls mitochondrial cristae structure in mouse muscle, influencing mtDNA replication and cholesterol levels. J Cell Sci. 2018 Jul 4;131(13):jcs217075
• All: 5 reference(s)