Ly6a^{tm1.1(cre)Jmol}
Targeted Allele Detail

Summary

• Symbol: Ly6a^{tm1.1(cre)Jmol}
• Name: lymphocyte antigen 6 family member A; targeted mutation 1.1, Jeffery D Molkentin
• MGI ID: MGI:6198589
• Synonyms: Ly6a^Cre
• Gene: Ly6a Location: Chr15:74866726-74869880 bp, - strand Genetic Position: Chr15, 34.29 cM
• Alliance: Ly6a^{tm1.1(cre)Jmol} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264764
• Parent Cell Line: W4 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Recombinase)
• Mutation: Insertion
• Ly6a^{tm1.1(cre)Jmol} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ly6a (MGI:107527)

• Mutation details: A cDNA encoding cre-IRES-EGFPnls (nuclear localized EGFP) as well as an FRT-flanked neomycin cassette was recombined in-frame with the Ly6a ATG start codon (exon 2) via homologous recombination. Resultant mice were crossed with Flpe mice to delete the neomycin cassette. Though present in the targeting construct, IRES-driven expression of EGFPnls is not observed. Expression of Ly6a is eliminated by the targeted allele. (J:264764)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ly6a (mouse) Summary of all recombinase alleles driven by Ly6a. Comparative matrix view of recombinase activities Alliance mouse Ly6a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ly6a Mutation: 22 strains or lines available

References

• Original: J:264764 Vagnozzi RJ, et al., Genetic Lineage Tracing of Sca-1(+) Cells Reveals Endothelial but Not Myogenic Contribution to the Murine Heart. Circulation. 2018 Jul 10
• All: 1 reference(s)