Nphs2^{tm1.1(icre)Ptb}
Targeted Allele Detail

Summary

• Symbol: Nphs2^{tm1.1(icre)Ptb}
• Name: nephrosis 2, podocin; targeted mutation 1.1, Paul T Brinkkoetter
• MGI ID: MGI:6200320
• Synonyms: Nphs2^{pod.T2A.ciCre.T2A.mTomato}
• Gene: Nphs2 Location: Chr1:156138297-156155605 bp, + strand Genetic Position: Chr1, 67.71 cM, cytoband G3
• Alliance: Nphs2^{tm1.1(icre)Ptb} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264681
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (No functional change, Recombinase, Reporter)
• Mutation: Insertion
• Nphs2^{tm1.1(icre)Ptb} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Nphs2 (MGI:2157018)

• Mutation details: The targeting construct designed to insert a codon improved Cre Recombinase gene (icre) and a membrane-targeted tandem dimer tdTomato (mTomato) under the control of the endogenous Nphs2 promoter using viral T2A-peptides was generated using bacterial artificial chromosome clones from the C57BL/6J RPCIB-731 BAC library. The T2A.icre.T2A.mTomato cassette was inserted between the last codon of exon 8 of Nphs2 and the stop codon. A frt-flanked Puromycin resistance cassette was placed in intron between exon 7 and exon 8. The constitutive knock-in allele was generated after Puromycin resistance cassette was removed by in vivo Flp-mediated removal of the selection marker. (J:264681)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Nphs2 (mouse) Summary of all recombinase alleles driven by Nphs2. Comparative matrix view of recombinase activities Alliance mouse Nphs2 gene page

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nphs2 Mutation: 30 strains or lines available

References

• Original: J:264681 Koehler S, et al., Construction of a viral T2A-peptide based knock-in mouse model for enhanced Cre recombinase activity and fluorescent labeling of podocytes. Kidney Int. 2017 Jun;91(6):1510-1517
• All: 2 reference(s)