Emc3^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Emc3^{tm1c(EUCOMM)Wtsi}
• Name: ER membrane protein complex subunit 3; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6200599
• Synonyms: emc^fl
• Gene: Emc3 Location: Chr6:113491848-113508599 bp, - strand Genetic Position: Chr6, 52.78 cM
• Alliance: Emc3^{tm1c(EUCOMM)Wtsi} page
• IMPC: Emc3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265020
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 113496643 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 113498102. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:265020)

Expression

In Mice Carrying this Mutation:

106 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Emc3 Mutation: 18 strains or lines available

References

• Original: J:265020 Tang X, et al., EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. J Clin Invest. 2017 Dec 1;127(12):4314-4325
• All: 4 reference(s)