Hcrt^{tm1.1(cre)Ldl}
Targeted Allele Detail

Summary

• Symbol: Hcrt^{tm1.1(cre)Ldl}
• Name: hypocretin; targeted mutation 1, Luis de Lecea
• MGI ID: MGI:6201351
• Synonyms: Hcrt-Cre, Hcrt-IRES-Cre
• Gene: Hcrt Location: Chr11:100652519-100653757 bp, - strand Genetic Position: Chr11, 63.6 cM
• Alliance: Hcrt^{tm1.1(cre)Ldl} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264534
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Hcrt^{tm1.1(cre)Ldl} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hcrt (MGI:1202306)

• Mutation details: Bacterial artificial chromosome clones containing the preprohypocretin (Hcrt) gene were used to target a cassette containing the internal ribosomal entry sequence (IRES) linked to Cre recombinase and a Frt-flanked neomycin resistance gene immediately downstream of the exon 2 stop codon. The target construct was electroporated into C57BL/6 embryonic stem cells, targeted clones were injected into blastocysts, and resultantchimeras were bred for germline transmission and then crossed with Flp-deletion mice to remove the neomycin selection gene. (J:264534)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hcrt (mouse) Summary of all recombinase alleles driven by Hcrt. Comparative matrix view of recombinase activities Alliance mouse Hcrt gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hcrt Mutation: 19 strains or lines available

References

• Original: J:264534 Giardino WJ, et al., Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states. Nat Neurosci. 2018 Aug;21(8):1084-1095
• All: 6 reference(s)