Iws1^tm1.1Cop
Targeted Allele Detail

Summary

• Symbol: Iws1^tm1.1Cop
• Name: IWS1, SUPT6 interacting protein; targeted mutation 1.1, Vincenzo Coppola
• MGI ID: MGI:6201357
• Synonyms: Iws1^tm1b
• Gene: Iws1 Location: Chr18:32200794-32237381 bp, + strand Genetic Position: Chr18, 17.92 cM, cytoband B3
• Alliance: Iws1^tm1.1Cop page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265507
• Parent Cell Line: S1B6 (ES Cell)
• Strain of Origin: 129S1/SvImJ or C57BL/6J

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 32215869 of Chromosome 18 upstream of the exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 4 at position 32216763. Exon 4 is thus flanked by loxP sites. Cre-mediated recombination without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:265507)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Iws1 Mutation: 50 strains or lines available

References

• Original: J:265507 Orlacchio A, et al., Genetic ablation of interacting with Spt6 (Iws1) causes early embryonic lethality. PLoS One. 2018;13(9):e0201030
• All: 1 reference(s)