Slc9a9^{tm2d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc9a9^{tm2d(KOMP)Wtsi}
• Name: solute carrier family 9 (sodium/hydrogen exchanger), member 9; targeted mutation 2d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6201444
• Gene: Slc9a9 Location: Chr9:94551962-95112498 bp, + strand Genetic Position: Chr9, 49.22 cM
• Alliance: Slc9a9^{tm2d(KOMP)Wtsi} page
• IMPC: Slc9a9 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258593
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 94691494 of Chromosome 9 upstream of the exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA cassette, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 94692377. Exon 5 is thus flanked by loxP sites. Flp mediated recombination created a "conditional ready" (floxed) allele. Subsequent cre expression created a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:258593)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc9a9 Mutation: 42 strains or lines available

References

• Original: J:258593 Ullman JC, et al., A mouse model of autism implicates endosome pH in the regulation of presynaptic calcium entry. Nat Commun. 2018 Jan 23;9(1):330
• All: 1 reference(s)