Tg(Chrna5-cre)2Et
Transgene Detail

Summary

• Symbol: Tg(Chrna5-cre)2Et
• Name: transgene insertion 2, Eric E Turner
• MGI ID: MGI:6201567
• Transgene: Tg(Chrna5-cre)2Et Location: unknown
• Alliance: Tg(Chrna5-cre)2Et page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Chrna5-cre)2Et expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Chrna5 (MGI:87889)

• Mutation details: A cre-expression cassette, followed by a polyadenylation sequence, was inserted into BAC clone RP24-318N16 at the initiating ATG codon of the first coding exon of the Chrna5 gene so that cre expression is driven by the regulatory sequences of the BAC gene. This BAC clone generated by the Gene Expression Nervous System Atlas (GENSAT) Project was furtehr subcloned to include only Chrna5 and deleting Chrna3 and Chrna4 genes on opposit strand of the original BAC clone. The resulting BAC was used to generate three independent lines. Transgenic line 2 was used for expression analyses. (J:264332)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Chrna5 (mouse) Summary of all recombinase alleles driven by Chrna5. Comparative matrix view of recombinase activities Alliance mouse Chrna5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:264332 Morton G, et al., Chrna5-expressing neurons in the interpeduncular nucleus mediate aversion primed by prior stimulation or nicotine exposure. J Neurosci. 2018 Jun 28
• All: 1 reference(s)