Toa1^MRL/MP
QTL Variant Detail

Summary

• QTL variant: Toa1^MRL/MP
• Name: testicular oocyte appearance 1; MRL/MPJ
• MGI ID: MGI:6201619
• QTL: Toa1 Location: Chr15:20807352-28494067 bp Genetic Position: Chr15, Syntenic

Variant origin

• Strain of Specimen: MRL/MpJ

Variant description

• Allele Type: QTL
• Inheritance: Not Specified

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:191336

Mammals produce sperm or oocytes depending on their sex; however, newborn MRL/MpJ male mice produce oocytes within their testes.

In order to identify genes involved in testicular oocyte appearance in newborn MRL/MpJ mice, QTL analysis was performed using 266 MBM mice - N2 backcross progeny from crosses between female (MRL/MpJ x C57BL/6)F1 and male MRL/MpJ mice.

Genomic DNA was prepared from the spleen of each MBM mouse using a standard protocol. In total, 113 microsatellite markers identified using Mouse Genome Informatics (MGI) were used for a genome-wide scan with a mean intermarker distance of 10-20 cM to genotype the C57BL/6 or MRL/MpJ allele.

Two phenotypic categories were used: mice with testicular oocytes (value = 1) and mice without testicular oocytes (value = 0). Genotyping data and phenotype values were entered into the Map Manager QTXb20 program and analyzed.

QTL analysis revealed the presence of two susceptibility loci for the appearance of testicular oocytes on Chr 15:

QTL Toa1 (testicular oocyte appearance 1) maps to Chr15:8.23-11.93 cM (D15Mit130-D15Mit18) with an LRS of 13.2.

QTL Toa2 (testicular oocyte appearance 2) maps to Chr15:15.18-16.73 cM (D15Mit1007-D15Mit87) with an LRS of 12.9.

Mice homozygous for the MRL/MpJ genotype had significantly higher oocyte scores than heterozygous mice for both QTL regions.

References

• Original: J:191336 Otsuka S, et al., Genomic analysis of the appearance of testicular oocytes in MRL/MpJ mice. Mamm Genome. 2012 Dec;23(11-12):741-8
• All: 1 reference(s)