Alb^{em1(dre/ERT2)Bzsh}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Alb^{em1(dre/ERT2)Bzsh}
• Name: albumin; endonuclease-mediated mutation 1, Bin Zhou
• MGI ID: MGI:6202045
• Synonyms: Alb-DreER
• Gene: Alb Location: Chr5:90608756-90624461 bp, + strand Genetic Position: Chr5, 44.7 cM
• Alliance: Alb^{em1(dre/ERT2)Bzsh} page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Alb^{em1(dre/ERT2)Bzsh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Alb (MGI:87991)

• Mutation details: The line was generated by inserting a cDNA encoding IRES-Dre recombinase fused with a mutant form of estrogen receptor hormon-binding domaine C (ERT2) into in-frame with the translational termination codon of the last coding exon of Alb by homologous recombination using the CRISPR/Cas9 technology. This knock-in strategy leads to simultaneous expression of DreER and endogenous albumin. (J:263751)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Alb (mouse) Summary of all recombinase alleles driven by Alb. Comparative matrix view of recombinase activities Alliance mouse Alb gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Alb Mutation: 97 strains or lines available

References

• Original: J:263751 Wang Y, et al., Genetic tracing of hepatocytes in liver homeostasis, injury, and regeneration. J Biol Chem. 2017 May 26;292(21):8594-8604
• All: 3 reference(s)