P2rx7^{tm1.1(P2RX7)Jde}
Targeted Allele Detail

Summary

• Symbol: P2rx7^{tm1.1(P2RX7)Jde}
• Name: purinergic receptor P2X, ligand-gated ion channel, 7; targeted mutation 1.1, Jan Deussing
• MGI ID: MGI:6203041
• Synonyms: P2rx7^hP2RX7, P2rx7^hWT
• Gene: P2rx7 Location: Chr5:122781974-122829495 bp, + strand Genetic Position: Chr5, 62.5 cM, cytoband F
• Alliance: P2rx7^{tm1.1(P2RX7)Jde} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244726
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The targeting vector consists from 5' to 3': a loxP site followed by the 3'-end (1.4-kb) of mouse intron 1; the murine exon 2 replaced by the human P2RX7 cDNA comprising exons 2-13; a reverse oriented selection marker flanked by frt sites which consists of a phosphoglycerate kinase (PGK) promoter driven neomycin resistance gene equipped with a bovine growth hormone (bGH) poly A signal (pA), a second loxP site and a quadruple poly A signal consisting of a bGHpA, a PGK pA, and 2 SV40 pAs. After germ-line transmission of the modified allele via homologous recombination in ES cells, offspring from male chimeras are bred. The FRT flanked neo cassette was removed by breeding to deleter-flp mice. (J:244726)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P2rx7 Mutation: 54 strains or lines available

References

• Original: J:244726 Metzger MW, et al., Genetically dissecting P2rx7 expression within the central nervous system using conditional humanized mice. Purinergic Signal. 2017 Jun;13(2):153-170
• All: 4 reference(s)