Gt(ROSA)26Sor^{tm2.1(CAG-APX1*)Ddg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-APX1*)Ddg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, David D Ginty
• MGI ID: MGI:6203624
• Synonyms: ROSA26^{LSL-ER-dAPEX2}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-APX1*)Ddg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280094
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: A CAG promoter, FRT-flanked Stop cassette, loxP-flanked Stop cassette (in reverse transcriptional orientation), ER-dAPEX2, WPRE, bGHpA, attB, PGK-Neo, PGKpA, and attP were introduced to intron 1 via homologous recombination. APEX2 is an enhanced (K14D, W41F, E112K, A134P) soybean ascorbate peroxidase (APX1) that is less catalytically active than horseradish peroxidase (HRP), but is functional when expressed in cytosolic environments, unlike HRP. Dimeric APEX2 (dAPEX2) contains the reversion of K14 and E112 and is presumed to enhance heme affinity and thermal stability of the protein. Targeting of dAPEX2 to the endoplasmic reticulum to create ER-dAPEX2 (Addgene plasmid 117182) was accomplished by a an N-terminal Igk signal sequence and C-terminal KDEL retention sequence. Flp-mediated recombination removed the FRT-flanked stop cassette and left the floxed stop cassette intact. (J:280094)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1040 strains or lines available

References

• Original: J:280094 Zhang Q, et al., Multiplexed peroxidase-based electron microscopy labeling enables simultaneous visualization of multiple cell types. Nat Neurosci. 2019 May;22(5):828-839
• All: 1 reference(s)