Ager^{tm2.1(cre/ERT2)Blh}
Targeted Allele Detail

Summary

• Symbol: Ager^{tm2.1(cre/ERT2)Blh}
• Name: advanced glycosylation end product-specific receptor; targeted mutation 2.1, Brigid L Hogan
• MGI ID: MGI:6203633
• Synonyms: Ager-CreER^T2
• Gene: Ager Location: Chr17:34816836-34819910 bp, + strand Genetic Position: Chr17, 18.18 cM
• Alliance: Ager^{tm2.1(cre/ERT2)Blh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265836
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inducible, Null/knockout, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Ager^{tm2.1(cre/ERT2)Blh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ager (MGI:893592)

• Mutation details: The targeting vector was designed to insert a cre/ERT2 fusion gene, followed by a polyadenylation signal and a frt-flanked neomycin resistance cassette, into the start codon of Ager gene. The construct was electroporated into ES cells for homologous recombination. Correctly targeted ES cells were injected into C57BL/6 blastocysts and resulting chimeric mice were bred with FLP-deleter mice to remove the neo cassette. It is a null allele. Ager protein is not detected in lungs of the homozygous mice. (J:265836)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ager (mouse) Summary of all recombinase alleles driven by Ager. Comparative matrix view of recombinase activities Alliance mouse Ager gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ager Mutation: 29 strains or lines available

References

• Original: J:265836 Chung MI, et al., Ager-CreER(T2): A New Genetic Tool for Studying Lung Alveolar Development, Homeostasis, and Repair. Am J Respir Cell Mol Biol. 2018 Jul 16
• All: 9 reference(s)