Actb^em1Erv
Endonuclease-mediated Allele Detail

Summary

• Symbol: Actb^em1Erv
• Name: actin, beta; endonuclease-mediated mutation 1, James Ervasti
• MGI ID: MGI:6209780
• Synonyms: Actb^c-g
• Gene: Actb Location: Chr5:142888870-142892509 bp, - strand Genetic Position: Chr5, 81.8 cM
• Alliance: Actb^em1Erv page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: TALEN-based genome editing was used to introduce 8 nucleotide alterations, which function to exchange the translation of beta-actin D2-4, V10 to gamma-actin E2-4, I10, and to disrupt TALEN re-binding to the edited locus. Mice are null for the beta-actin protein. (J:265862)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Actb Mutation: 53 strains or lines available

References

• Original: J:265862 Patrinostro X, et al., Essential nucleotide- and protein-dependent functions of Actb/beta-actin. Proc Natl Acad Sci U S A. 2018 Jul 31;115(31):7973-7978
• All: 1 reference(s)