Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich
Transgene Detail

Summary

• Symbol: Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich
• Name: transgene insertion 1, Colin G Nichols
• MGI ID: MGI:6236321
• Transgene: Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich Location: unknown
• Alliance: Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(ACTB-EGFP,-Kcnj8*Q53R*G343D)1Nich expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Kcnj8 (MGI:1100508)

• Mutation details: This transgene consists of the rat Kcnj8 cDNA with a glutamine to arginine substation at amino acid 53 (Gly343Asp) and a glycine to aspartate substitution at amino acid 343 (Gly343Asp), rendering ATP-insensitivity, under the control of the CX1 promoter which contains the chicken beta-actin promoter followed by the enhanced green fluorescent protein coding region (including a stop codon) flanked by loxP sites. EGFP is ubiquitously expressed unless cre-recombinase is expressed which excises the EGFP and results in expression of the mutant Kcnj8. Lines 1 and 2 are the high expressing lines, with line 1 entered as the representative line. (J:202037)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:202037 Li A, et al., Hypotension due to Kir6.1 gain-of-function in vascular smooth muscle. J Am Heart Assoc. 2013 Aug;2(4):e000365
• All: 2 reference(s)