Gt(ROSA)26Sor^{tm1(CAG-C9orf72)Lgu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-C9orf72)Lgu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Leonard Guarente
• MGI ID: MGI:6241353
• Synonyms: R26-CAG-LSL-C9orf72
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-C9orf72)Lgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-C9orf72)Lgu} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	C9orf72 (MGI:1920455)

• Mutation details: The targeting vector is designed to have (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP site, a transcriptional/translational STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a second loxP site, a cDNA sequence encoding the mouse C9orf72 member of C9orf72-SMCR8 complex (C9orf72), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), a BGH polyA signal, a second FRT site and a neo cassette. The construct is inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 958 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)