Rsph6a^em2Osb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rsph6a^em2Osb
• Name: radial spoke head 6 homolog A (Chlamydomonas); endonuclease-mediated mutation 2, Osaka University
• MGI ID: MGI:6241362
• Synonyms: Rsph6a^LD
• Gene: Rsph6a Location: Chr7:18788615-18808372 bp, + strand Genetic Position: Chr7, 9.46 cM, cytoband A2
• Alliance: Rsph6a^em2Osb page

Mutation origin

• Strain of Origin: (C57BL/6NJcl x DBA/2NJcl)F2

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination created a 12,068 bp deletion spanning from exon 1 to exon 4. This deletion removes amino acids 122-542 of the long isoform (variant 1) and amino acids 122-289 of the short isoform (variant 2). Western blot analysis confirmed the absence of expression in homozygous mice. (J:266136)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Rsph6a Mutation: 36 strains or lines available

References

• Original: J:266136 Abbasi F, et al., RSPH6A is required for sperm flagellum formation and male fertility in mice. J Cell Sci. 2018 Oct 11;131(19):jcs221648
• All: 1 reference(s)