Igh^{tm1.1(PAX5/ELN)Cbd}
Targeted Allele Detail

Summary

• Symbol: Igh^{tm1.1(PAX5/ELN)Cbd}
• Name: immunoglobulin heavy chain complex; targeted mutation 1.1, Cyril Broccardo
• MGI ID: MGI:6251554
• Synonyms: PAX5-ELN, PE^tg
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266607
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Igh^{tm1.1(PAX5/ELN)Cbd} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ELN (2006)

  Eln	(MGI:95317)

  cDNA fusion of the 5' end of human PAX5 (exons 1 through 7) with the coding sequence of human ENL (exons 2 through 33)

  human

  PAX5 (5079)

  Pax5	(MGI:97489)

  cDNA fusion of the 5' end of human PAX5 (exons 1 through 7) with the coding sequence of human ENL (exons 2 through 33)

• Mutation details: The knock-in contruct inserted downstream of the Emu enhancer contains a VH promoter driving B cell expression of the cDNA fusion of the 5' end of human PAX5 (exons 1 through 7) with the coding sequence of human ENL (exons 2 through 33). This fusion is present in B cell acute lymphoblastic leukemia with the translocation t(7;9)(q11;p13). Cre-mediated recombination removed the floxed neomycin resistance cassette. (J:266607)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 39 strains or lines available

References

• Original: J:266607 Jamrog L, et al., PAX5-ELN oncoprotein promotes multistep B-cell acute lymphoblastic leukemia in mice. Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10357-10362
• All: 1 reference(s)