Trpv4^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Trpv4^{tm1d(KOMP)Wtsi}
• Name: transient receptor potential cation channel, subfamily V, member 4; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6256768
• Gene: Trpv4 Location: Chr5:114760213-114796482 bp, - strand Genetic Position: Chr5, 55.99 cM, cytoband F
• Alliance: Trpv4^{tm1d(KOMP)Wtsi} page
• IMPC: Trpv4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266383
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 114772456 of Chromosome 5 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 114773304. The critical exon 6 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:266383)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trpv4 Mutation: 57 strains or lines available

References

• Original: J:266383 Matsumoto H, et al., Retinal Detachment-Induced Muller Glial Cell Swelling Activates TRPV4 Ion Channels and Triggers Photoreceptor Death at Body Temperature. J Neurosci. 2018 Oct 10;38(41):8745-8758
• All: 1 reference(s)