Slc1a4^{tm1e(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc1a4^{tm1e(KOMP)Wtsi}
• Name: solute carrier family 1 (glutamate/neutral amino acid transporter), member 4; targeted mutation 1e, Wellcome Trust Sanger Institute
• MGI ID: MGI:6256798
• Gene: Slc1a4 Location: Chr11:20252180-20282713 bp, - strand Genetic Position: Chr11, 12.97 cM
• Alliance: Slc1a4^{tm1e(KOMP)Wtsi} page
• IMPC: Slc1a4 gene page

Mutation origin

• Mutant Cell Line: EPD0133_1_A03
• Germline Transmission: Earliest citation of germline transmission: J:266681
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 20264513 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534, J:266681)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Slc1a4 Mutation: 41 strains or lines available

References

• Original: J:266681 Kaplan E, et al., ASCT1 (Slc1a4) transporter is a physiologic regulator of brain d-serine and neurodevelopment. Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9628-9633
• All: 6 reference(s)