Col1a1^{tm1(tetO-GFP/RNAi:Stat3)Ern}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-GFP/RNAi:Stat3)Ern}
• Name: collagen, type I, alpha 1; targeted mutation 1, Matthias Ernst
• MGI ID: MGI:6257037
• Synonyms: TRE-Stat3.1348
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-GFP/RNAi:Stat3)Ern} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239149
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: RMCE (recombinase-mediated cassette exchange) was utilized to insert tetO-GFP/RNAi:Stat3 into the Col1a1 locus. KH2 embryonic stem cells (C57BL/6 x 129S4Sv/Jae), carrying the Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} allele, were previously re-targeted to insert an FRT site-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette in the 3' UTR of the Col1a1 locus, thus adapting the ES cell line for RMCE. The vector contains a tetracycline responsive promoter (TRE;tetO) controlling expression of a GFP reporter and short-hairpin RNA sequence targeting Stat3 embedded in the Mir30 structure. (J:239149)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 163 strains or lines available

Notes

This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

• Original: J:239149 Alorro MG, et al., Generation of an inducible mouse model to reversibly silence Stat3. Genesis. 2017 Apr;55(4)
• All: 1 reference(s)