Adarb2^{tm1b(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Adarb2^{tm1b(KOMP)Mbp}
• Name: adenosine deaminase, RNA-specific, B2; targeted mutation 1b, Mouse Biology Program, UC Davis
• MGI ID: MGI:6257878
• Synonyms: Adar3^exon3
• Gene: Adarb2 Location: Chr13:8252902-8818783 bp, + strand Genetic Position: Chr13, 3.48 cM, cytoband A1
• Alliance: Adarb2^{tm1b(KOMP)Mbp} page
• IMPC: Adarb2 gene page

Mutation origin

• Mutant Cell Lines: DEPD0006_14_A03, DEPD0006_14_A05
• Germline Transmission: Earliest citation of germline transmission: J:264218
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 8619244 of Chromosome 13 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 8620832. The critical exon 3 is thus flanked by loxP sites. Cre expression without flp expression created a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:264218)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Adarb2 Mutation: 58 strains or lines available

References

• Original: J:264218 Mladenova D, et al., Adar3 Is Involved in Learning and Memory in Mice. Front Neurosci. 2018;12:243
• All: 1 reference(s)