Tg(KRT5-Terf2)POBlas
Transgene Detail

Summary

• Symbol: Tg(KRT5-Terf2)POBlas
• Name: transgene insertion PO, Maria A Blasco
• MGI ID: MGI:6258222
• Synonyms: PO K5-Terf2
• Transgene: Tg(KRT5-Terf2)POBlas Location: unknown
• Alliance: Tg(KRT5-Terf2)POBlas page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(KRT5-Terf2)POBlas expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Terf2 (MGI:1195972)

• Tg(KRT5-Terf2)POBlas expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  cattle	KRT5 (281268)

• Mutation details: The full-length mouse Terf2 cDNA was placed under control of the 5' regulatory region of the bovine keratin K5 promoter, which targets TERF2 to basal cells and stem cells of the epidermis. Three transgenic lines were generated and analyzed (PM, PN and PO). In the PO line (males and females), the levels of Terf2 mRNA expression in tail and back skin were ~23 times greater than those in wild-type controls. Western blot analysis revealed significantly elevated TERF2 protein levels in primary keratinocytes derived from PO transgenic mice. Combined immunofluorescence and telomeric Q-FISH analysis showed that the overexpressed TERF2 protein localized to telomeres. (J:102653)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:102653 Munoz P, et al., XPF nuclease-dependent telomere loss and increased DNA damage in mice overexpressing TRF2 result in premature aging and cancer. Nat Genet. 2005 Oct;37(10):1063-71
• All: 1 reference(s)