Sh3rf3^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sh3rf3^em1(IMPC)J
• Name: SH3 domain containing ring finger 3; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6258587
• Gene: Sh3rf3 Location: Chr10:58649181-58974738 bp, + strand Genetic Position: Chr10, 29.41 cM
• Alliance: Sh3rf3^em1(IMPC)J page
• IMPC: Sh3rf3 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GTACCATCCCAAGGTCAACA and GAGAATGTCGACAGACATGA, which resulted in a 361 bp deletion beginning at Chromosome 10 position 59,071,696 bp and ending after 59,072,056 bp (GRCm38/mm10). This mutation deletes ENSMUSE00001288805 (exon 5) and 256 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 428 and early truncation 12 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Sh3rf3 Mutation: 51 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)