Tut4^tm3.1Doca
Targeted Allele Detail

Summary

• Symbol: Tut4^tm3.1Doca
• Name: terminal uridylyl transferase 4; targeted mutation 3.1, Donal O'Carroll
• MGI ID: MGI:6259531
• Synonyms: Tut4^AAD
• Gene: Tut4 Location: Chr4:108316623-108416618 bp, + strand Genetic Position: Chr4, 50.55 cM
• Alliance: Tut4^tm3.1Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262343
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 15 was replaced with a modified one in which the codons for Asp1026 and Asp1028 were mutated to encode alanine. These mutations result in an enzymatically inactive protein. Flp-mediated recombination removed the selection cassette inserted downstream of the modified exon 15. (J:262343)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tut4 Mutation: 81 strains or lines available

References

• Original: J:262343 Morgan M, et al., mRNA 3' uridylation and poly(A) tail length sculpt the mammalian maternal transcriptome. Nature. 2017 Aug 17;548(7667):347-351
• All: 2 reference(s)