Brs3^{tm3.1(cre/ERT2)Rei}
Targeted Allele Detail

Summary

• Symbol: Brs3^{tm3.1(cre/ERT2)Rei}
• Name: bombesin-like receptor 3; targeted mutation 3.1, Marc L Reitman
• MGI ID: MGI:6259727
• Synonyms: Brs3-2A-CreERT2, Brs3-Cre, BRS3-CreER
• Gene: Brs3 Location: ChrX:56088434-56094118 bp, + strand Genetic Position: ChrX, 30.78 cM, cytoband A7.1-A7.2
• Alliance: Brs3^{tm3.1(cre/ERT2)Rei} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:271513
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Hypomorph, Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Brs3^{tm3.1(cre/ERT2)Rei} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Brs3 (MGI:1100501)

• Mutation details: A targeting construct containing a 2A peptide, CreERT2 fusion gene, thymidine kinase polyadenylation signal sequence, followed by a FRT site flanked neo cassette was inserted into exon 3 of the targeted Brs3 gene into embryonic stem (ES) cells. The resulting chimeric animals were tested for germline transmission and were then bred to FLPo recombinase expressing mice to remove the neo cassette. (J:101977, J:271513)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Brs3 (mouse) Summary of all recombinase alleles driven by Brs3. Comparative matrix view of recombinase activities Alliance mouse Brs3 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Brs3 Mutation: 14 strains or lines available

References

• Original: J:271513 Pinol RA, et al., Brs3 neurons in the mouse dorsomedial hypothalamus regulate body temperature, energy expenditure, and heart rate, but not food intake. Nat Neurosci. 2018 Nov;21(11):1530-1540
• All: 4 reference(s)