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Pold4em1(IMPC)J
Endonuclease-mediated Allele Detail
Summary
Symbol: Pold4em1(IMPC)J
Name: polymerase (DNA-directed), delta 4; endonuclease-mediated mutation 1, Jackson
MGI ID: MGI:6259891
Gene: Pold4  Location: Chr19:4281953-4283688 bp, + strand  Genetic Position: Chr19, 3.93 cM
Alliance: Pold4em1(IMPC)J page
IMPC: Pold4 gene page
Mutation
origin
Strain of Origin:  C57BL/6NJ
Project Collection: IMPC
Mutation
description
Allele Type:    Endonuclease-mediated (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation detailsThis allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences GCTGGATGGCAAAGGCAAAT and CCCTACGTAGACCAAGAGGG, which resulted in a 2-part deletion beginning at Chromosome 19 position 4,232,361 bp for 446 bp, followed by 5 bp of retained sequence (TGGGT), then an additional 110 bp deletion that ends after 4,232,921 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000145535 and ENSMUSE00000145533 (exons 2 and 3) and 355 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 32 and early truncation 20 amino acids later. (J:188991)
Inheritance:    Not Specified
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Pold4 Mutation:  10 strains or lines available
References
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory