Tg(Tyr-HRAS*G12V)#Acc
Transgene Detail

Summary

• Symbol: Tg(Tyr-HRAS*G12V)#Acc
• Name: transgene insertion, Arizona Cancer Center
• MGI ID: MGI:6266855
• Synonyms: TP-ras
• Transgene: Tg(Tyr-HRAS*G12V)#Acc Location: unknown
• Alliance: Tg(Tyr-HRAS*G12V)#Acc page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Constitutively active, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tyr-HRAS*G12V)#Acc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HRAS (3265)

  Hras	(MGI:96224)

  mouse tyrosinase (Tyr) promoter drives expression of the activated human HRAS gene

• Mutation details: A 2.5-kb promoter region from the mouse tyrosinase (Tyr) gene was used to drive expression of the activated human HRAS gene (T24 Ha-ras), containing a single amino acid substitution of a glycine to valine at codon 12, in pigment-producing cells. Two independent founder mice carrying the transgene survived and showed the same distinct phenotype. One of these founders, a male, showed a single or low copy number integration event. The offspring of this male founder were developed into a line that stably expressed the same phenotype. RT-PCR analysis revealed transgene expression in adult skin, brain and spleen tissue. No line number was specified, as denoted by the pound symbol (#). (J:90877)

Expression

In Structures Affected by this Mutation:

13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:90877 Powell MB, et al., Hyperpigmentation and melanocytic hyperplasia in transgenic mice expressing the human T24 Ha-ras gene regulated by a mouse tyrosinase promoter. Mol Carcinog. 1995 Feb;12(2):82-90
• All: 3 reference(s)