Als2^tm1Dwc
Targeted Allele Detail

Summary

• Symbol: Als2^tm1Dwc
• Name: alsin Rho guanine nucleotide exchange factor; targeted mutation 1, Don W Cleveland
• MGI ID: MGI:6266856
• Gene: Als2 Location: Chr1:59202085-59276390 bp, - strand Genetic Position: Chr1, 29.33 cM
• Alliance: Als2^tm1Dwc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267265
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin selection cassette replaced most of exon 4 and a portion of intron 4, producing a translation product of 171 amino acids followed by 3 unique residues and a premature stop codon at amino acid 175. Immunoblot of brain lysates indicates no detectable full-length protein or short forms of the protein in homozygotes. (J:267265)

Expression

In Mice Carrying this Mutation:

4 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Als2 Mutation: 88 strains or lines available

References

• Original: J:267265 Yamanaka K, et al., Progressive spinal axonal degeneration and slowness in ALS2-deficient mice. Ann Neurol. 2006 Jul;60(1):95-104
• All: 1 reference(s)