Pls3^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pls3^{tm1d(EUCOMM)Wtsi}
• Name: plastin 3 (T-isoform); targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6266858
• Gene: Pls3 Location: ChrX:74829260-74918788 bp, - strand Genetic Position: ChrX, 38.3 cM
• Alliance: Pls3^{tm1d(EUCOMM)Wtsi} page
• IMPC: Pls3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267292
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 74848486 of Chromosome X upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 74849958. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267292)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pls3 Mutation: 20 strains or lines available

References

• Original: J:267292 Neugebauer J, et al., Plastin 3 influences bone homeostasis through regulation of osteoclast activity. Hum Mol Genet. 2018 Dec 15;27(24):4249-4262
• All: 2 reference(s)