Marchf3^em1Hbsh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Marchf3^em1Hbsh
• Name: membrane associated ring-CH-type finger 3; endonuclease-mediated mutation 1, Hong-Bing Shu
• MGI ID: MGI:6269174
• Synonyms: March3^-
• Gene: Marchf3 Location: Chr18:56894788-57058587 bp, - strand Genetic Position: Chr18, 30.92 cM, cytoband D2
• Alliance: Marchf3^em1Hbsh page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exons 1 and 2 were targeted with respective sgRNAs using CRISPR/Cas9 technology. This resulted in the loss of coding nucleotides 65-297 spanning exons 1 and 2. The deletion causes a frameshift and premature translation stop codon, creating a knockout allele. (J:267641)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Marchf3 Mutation: 17 strains or lines available

References

• Original: J:267641 Lin H, et al., MARCH3 attenuates IL-1beta-triggered inflammation by mediating K48-linked polyubiquitination and degradation of IL-1RI. Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):12483-12488
• All: 1 reference(s)