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Rnpc3tm1c(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Rnpc3tm1c(EUCOMM)Wtsi
Name: RNA-binding region (RNP1, RRM) containing 3; targeted mutation 1c, Wellcome Trust Sanger Institute
MGI ID: MGI:6269405
Synonyms: Rnpc3lox
Gene: Rnpc3  Location: Chr3:113398716-113423798 bp, - strand  Genetic Position: Chr3, 49.35 cM, cytoband G1
Alliance: Rnpc3tm1c(EUCOMM)Wtsi page
IMPC: Rnpc3 gene page
Mutation
origin
Mutant Cell Line:  EPD0441_1_B10
Germline Transmission:  Earliest citation of germline transmission: J:267519
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready)
Mutation:    Insertion     Vector: L1L2_Pgk_P
 
Mutation detailsThe L1L2_Pgk_P cassette was inserted at position 113415224 of Chromosome 3 upstream of the critical exons 4 and 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 113416571. Exons 4 and 5 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exons 4 and 5 flanked by loxP sites. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267519)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 15 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rnpc3 Mutation:  41 strains or lines available
References
Original:  J:267519 Doggett K, et al., Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice. RNA. 2018 Dec;24(12):1856-1870
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory