Rsf1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rsf1^{tm1c(EUCOMM)Wtsi}
• Name: remodeling and spacing factor 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6270386
• Synonyms: Rsf1^LoxP
• Gene: Rsf1 Location: Chr7:97229103-97341989 bp, + strand Genetic Position: Chr7, 53.55 cM
• Alliance: Rsf1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Rsf1 gene page

Mutation origin

• Mutant Cell Line: EPD0103_1_F12
• Germline Transmission: Earliest citation of germline transmission: J:267928
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 97288085 of Chromosome 7 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 4 at position 97288972. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267928)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rsf1 Mutation: 438 strains or lines available

References

• Original: J:267928 Min S, et al., Chromatin-remodeling factor, RSF1, controls p53-mediated transcription in apoptosis upon DNA strand breaks. Cell Death Dis. 2018 Oct 22;9(11):1079
• All: 1 reference(s)