Cdk1em1Nagy
Endonuclease-mediated Allele Detail
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Symbol: |
Cdk1em1Nagy |
Name: |
cyclin dependent kinase 1; endonuclease-mediated mutation 1, Andras Nagy |
MGI ID: |
MGI:6272328 |
Synonyms: |
Cdk1-TK |
Gene: |
Cdk1 Location: Chr10:69170976-69188742 bp, - strand Genetic Position: Chr10, 36.07 cM
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Alliance: |
Cdk1em1Nagy page
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Germline Transmission: |
Earliest citation of germline transmission:
J:268168
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Parent Cell Line: |
C2 (Nagy) (ES Cell)
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Strain of Origin: |
C57BL/6NTac
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Allele Type: |
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Endonuclease-mediated (Inserted expressed sequence, Reporter) |
Mutation: |
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Insertion
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Mutation details: The 3' end of the gene was targeted with a targeting vector and sgRNAs using CRISPR/Cas9 technology. The vector that was inserted just before the stop codon contained the following elements: sequence for the F2A self-cleaving peptide, the 5' end of a modified herpes simplex virus thymidine kinase gene (TK.007), 5' terminal repeat (TR) sequence, a neomycin resistance gene cassette, 3' TR sequence, the 3' end of the TK gene, sequence for the T2A self-cleaving peptide, and the mCherry red fluorescent protein marker gene. The neo cassette was removed through subsequent transposase-mediated excission, leaving an allele expressing a tri-cistronic gene (cyclin-dependent kinase 1 + thymidine kinase + mCherry) under the control of the host gene promoter. Cells expressing TK die when exposed to ganciclovir (GCV). This allele was used to create heterozygous mutant mice.
(J:268168)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Cdk1 Mutation: |
20 strains or lines available
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Original: |
J:268168 Liang Q, et al., Linking a cell-division gene and a suicide gene to define and improve cell therapy safety. Nature. 2018 Nov;563(7733):701-704 |
All: |
1 reference(s) |
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