Hesx1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hesx1^em1(IMPC)J
• Name: homeobox gene expressed in ES cells; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6274391
• Gene: Hesx1 Location: Chr14:26716373-26724286 bp, + strand Genetic Position: Chr14, 16.09 cM, cytoband A3-B
• Alliance: Hesx1^em1(IMPC)J page
• IMPC: Hesx1 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences CTACCATTCATTTTTCAGTG and AGCCAATAGTGACAACACAA, which resulted in a 490 bp deletion beginning at Chromosome 14 position 27,001,112 bp and ending after 27,001,601 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000249582 (exon 2) and 200 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 52 and early truncation 8 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Hesx1 Mutation: 14 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)