Spring1^em1Nki
Endonuclease-mediated Allele Detail

Summary

• Symbol: Spring1^em1Nki
• Name: SREBF pathway regulator in golgi 1; endonuclease-mediated mutation 1, The Netherlands Cancer Institute
• MGI ID: MGI:6274678
• Synonyms: C12ORF49_KO, Spring^-
• Gene: Spring1 Location: Chr5:118383292-118401179 bp, + strand Genetic Position: Chr5, 58.32 cM, cytoband F
• Alliance: Spring1^em1Nki page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR-targeting generated a knockout allele using 5'gRNA (5'-TGCCATCCGATGCAATGCGCAGG-3') upstream of exon 2 and 3'gRNA (5'-AGGCAAGTTGGGCGTACTGCTGG-3') downstream of exon 5 to delete 9938 bp. (J:287088)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spring1 Mutation: 15 strains or lines available

References

• Original: J:287088 Loregger A, et al., Haploid genetic screens identify SPRING/C12ORF49 as a determinant of SREBP signaling and cholesterol metabolism. Nat Commun. 2020 Feb 28;11(1):1128
• All: 1 reference(s)