Lctl^tm1.1Gwis
Targeted Allele Detail

Summary

• Symbol: Lctl^tm1.1Gwis
• Name: lactase-like; targeted mutation 1.1 Graeme Wistow
• MGI ID: MGI:6274975
• Synonyms: KLPH KO
• Gene: Lctl Location: Chr9:64024429-64045400 bp, + strand Genetic Position: Chr9, 34.5 cM
• Alliance: Lctl^tm1.1Gwis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269554
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1-7 were replaced with an frt-flanked neomycin selection cassette. The neomycin selection cassette was removed by FLP1-mediated recombination, leaving a single frt site in the final allele. Loss of mRNA and protein expression was confirmed by western blot and RNAseq experiments. (J:269554)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lctl Mutation: 33 strains or lines available

References

• Original: J:269554 Fan J, et al., The klotho-related protein KLPH (lctl) has preferred expression in lens and is essential for expression of clic5 and normal lens suture formation. Exp Eye Res. 2018 Apr;169:111-121
• All: 2 reference(s)