Tg(Myh6-Kcnj8*/EGFP)1Wco
Transgene Detail

Summary

• Symbol: Tg(Myh6-Kcnj8*/EGFP)1Wco
• Name: transgene insertion 1, William A Coetzee
• MGI ID: MGI:6275008
• Synonyms: alpha-MHC-Kir6.1-AAA-eGFP, Kir6.1-AAA
• Transgene: Tg(Myh6-Kcnj8*/EGFP)1Wco Location: unknown
• Alliance: Tg(Myh6-Kcnj8*/EGFP)1Wco page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Dominant negative, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(Myh6-Kcnj8*/EGFP)1Wco expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Kcnj8 (25472)

  Kcnj8

  (MGI:1100508)

  the subunit pore region is mutated to three alanines resulting in a dominant negative

• Mutation details: The alpha-myosin heavy chain promoter drives cardiac-specific expression of a dominant negative rat Kcnj8 mutant, in which the subunit pore region was mutated from glycine-phenylalanine-glycine to three alanines, with a COOH-terminal enhanced green fluorescent protein. RT-PCR and Western blotting confirmed expression of the transgene specifically in the heart. Several lines were made but only one was further analyzed. (J:116284)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:116284 Tong X, et al., Consequences of cardiac myocyte-specific ablation of KATP channels in transgenic mice expressing dominant negative Kir6 subunits. Am J Physiol Heart Circ Physiol. 2006 Aug;291(2):H543-51
• All: 2 reference(s)