Tg(ACTB-EGFP,-Kcnj8*)1Wco
Transgene Detail

Summary

• Symbol: Tg(ACTB-EGFP,-Kcnj8*)1Wco
• Name: transgene insertion 1, William A Coetzee
• MGI ID: MGI:6275065
• Synonyms: Tg[CX1-eGFP-Kir6.1-AAA]
• Transgene: Tg(ACTB-EGFP,-Kcnj8*)1Wco Location: unknown
• Alliance: Tg(ACTB-EGFP,-Kcnj8*)1Wco page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Conditional ready, Dominant negative, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Tg(ACTB-EGFP,-Kcnj8*)1Wco expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  rat

  Kcnj8 (25472)

  Kcnj8

  (MGI:1100508)

  the subunit pore region is mutated to three alanines resulting in a dominant negative

• Mutation details: This transgene consists of the chicken beta-actin (CX1) promoter, followed by a loxP-flanked EGFP gene including a stop codon, and a pore rat Kcnj8 dominant negative mutant in which glycine-phenylalanine-glycine was mutated to three alanines. EGFP is ubiquitously expressed unless cre-recombinase is expressed which excises the EGFP and results in expression of the mutant Kcnj8. Three lines were generated but only a single line was selected for further study. (J:134852)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:134852 Malester B, et al., Transgenic expression of a dominant negative K(ATP) channel subunit in the mouse endothelium: effects on coronary flow and endothelin-1 secretion. FASEB J. 2007 Jul;21(9):2162-72
• All: 5 reference(s)