Btg4^em3Hyfn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Btg4^em3Hyfn
• Name: BTG anti-proliferation factor 4; endonuclease-mediated mutation 3, Heng-Yu Fan
• MGI ID: MGI:6276547
• Synonyms: Btg4^W95A
• Gene: Btg4 Location: Chr9:51013486-51031224 bp, + strand Genetic Position: Chr9, 27.84 cM
• Alliance: Btg4^em3Hyfn page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR/Cas9 mutagenesis was used to create mutations in codon 95 to alter the codon to encode an alanine instead of a tryptophan (W95A). This mutation was shown in vitro to abolish interactions with Cnot7 and Cnot8. An altered protein is detectable in oocytes derived from mutant mice. (J:245369)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Btg4 Mutation: 11 strains or lines available

References

• Original: J:245369 Yu C, et al., BTG4 is a meiotic cell cycle-coupled maternal-zygotic-transition licensing factor in oocytes. Nat Struct Mol Biol. 2016 May;23(5):387-94
• All: 1 reference(s)