Dok4^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dok4^em1(IMPC)J
• Name: docking protein 4; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6278523
• Gene: Dok4 Location: Chr8:95590456-95602940 bp, - strand Genetic Position: Chr8, 46.93 cM, cytoband C5
• Alliance: Dok4^em1(IMPC)J page
• IMPC: Dok4 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTGCGACCTACTCCACTAA and GCTTAGAGAAAACCTTAGGA, which resulted in a 625 bp deletion beginning at Chromosome 8 position 94,867,008 bp and ending after 94,867,632 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000287473 and ENSMUSE00000287448 (exons 3 and 4) and 402 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 23 and early truncation 36 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Dok4 Mutation: 13 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)