Map3k3^tm1.2Mlkn
Targeted Allele Detail

Summary

• Symbol: Map3k3^tm1.2Mlkn
• Name: mitogen-activated protein kinase kinase kinase 3; targeted mutation 1.2, Mark Kahn
• MGI ID: MGI:6279294
• Gene: Map3k3 Location: Chr11:105975439-106046272 bp, + strand Genetic Position: Chr11, 68.89 cM
• Alliance: Map3k3^tm1.2Mlkn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238897
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 9. An F3-flanked puromycin resistance cassette with a 3' loxP site was inserted downstream of exon 15. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination deleted exons 9-15. (J:238897)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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cn1	Map3k3^tm1.1Mlkn/Map3k3^tm1.2Mlkn Nfatc1^tm1.1(cre)Bz/Nfatc1^+	involves: 129S1/Sv * 129X1/SvJ * C57BL/6NTac

Phenotypes:

Affected Systems	cn1
show or hide all annotated terms
cardiovascular system	√
thin myocardium	√
mortality/aging	√
embryonic lethality during organogenesis	√
muscle	√
thin myocardium	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map3k3 Mutation: 27 strains or lines available

Notes

ES cell line = Art B6.3.5 (C57BL/6NTac)

References

• Original: J:238897 Zhou Z, et al., The cerebral cavernous malformation pathway controls cardiac development via regulation of endocardial MEKK3 signaling and KLF expression. Dev Cell. 2015 Jan 26;32(2):168-80
• All: 1 reference(s)