Mettl21c^tm1.1Krug
Targeted Allele Detail

Summary

• Symbol: Mettl21c^tm1.1Krug
• Name: methyltransferase 21C, AARS1 lysine; targeted mutation 1.1, Marcus Kruger
• MGI ID: MGI:6279318
• Synonyms: Mettl21c^-
• Gene: Mettl21c Location: Chr1:44048568-44059194 bp, - strand Genetic Position: Chr1, 23.52 cM
• Alliance: Mettl21c^tm1.1Krug page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:270838
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (C57BL/6 x 129)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector replacing exons 2-5 with a lacZ cassette and a loxP-flanked neomycin resistance cassette was obtained from Velocigene. Cre-mediated recombination removed the neomycin cassette leaving behind the inserted lacZ reporter sequence. Successful deletion was confirmed by PCR, immunoblotting of gluteus lysates, and mass spectrometry using an in vivo stable isotope labeling by amino acids in cell culture (SILAC) approach. (J:270838)

Expression

In Mice Carrying this Mutation:	47 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mettl21c Mutation: 14 strains or lines available

References

• Original: J:270838 Wiederstein JL, et al., Skeletal Muscle-Specific Methyltransferase METTL21C Trimethylates p97 and Regulates Autophagy-Associated Protein Breakdown. Cell Rep. 2018 May 1;23(5):1342-1356
• All: 2 reference(s)