E130308A19Rik^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: E130308A19Rik^em1(IMPC)J
• Name: RIKEN cDNA E130308A19 gene; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6281341
• Gene: E130308A19Rik Location: Chr4:59626116-59757649 bp, + strand Genetic Position: Chr4, 32.73 cM
• Alliance: E130308A19Rik^em1(IMPC)J page
• IMPC: E130308A19Rik gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TCTTATCCTGTGTGGACAGT and GCAGAATTCTTAGGTACGCG, which resulted in a 1432 bp deletion beginning at Chromosome 4 position 59,689,958 bp and ending after 59,691,389 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000339543 (exon 2) and 237 bp of flanking intronic sequence including the translation start and is predicted to result in a null allele. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any E130308A19Rik Mutation: 36 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)